Which of the following is a high-level disinfection method?

High-level disinfection methods are designed to eliminate a wide range of pathogens, including bacteria, viruses, and fungi, from reusable medical instruments. Hydrogen peroxide is recognized as a high-level disinfectant because it effectively kills a broad spectrum of microorganisms. It operates through the generation of reactive oxygen species, which destroy cellular components of pathogens.

When used in the appropriate concentrations, hydrogen peroxide can effectively disinfect surfaces and instruments. Its versatility and efficacy in disrupting the integrity of microbial cell walls make it a reliable choice in settings where high-level disinfection is required. This property is what categorizes it among other disinfectants as highly effective in preventing infections in clinical settings.

While boiling water can eliminate some pathogens, it does not meet the requirements for high-level disinfection as it may not be effective against all spores and certain resistant forms of bacteria. Chlorine bleach solutions can serve as high-level disinfectants, but their specific efficacy varies based on concentration, contact time, and organic load. UV light exposure is also a disinfection method, primarily effective for air and surface disinfection, but it does not typically achieve high-level disinfection standards on its own without proper protocols.